Players were met with boos as they knelt in support of the Black Lives Matter movement before Cambridge United's match against Colchester United on Tuesday night.
© Sky News Screen GrabPlayers take a knee at Tuesday's Cambridge v Colchester match
Boos were clearly audible from the stands at the Abbey Stadium in Cambridge, but fellow spectators quickly responded by applauding before the League Two match got under way.
The incident followed a similar one at Millwall earlier this month.
Sport stars, government ministers and club managers condemned Millwall fans, with bosses saying they were "dismayed and saddened", when crowds booed players for taking the knee before the match against Derby on 5 December.
© ImagebridgeFans booed Millwall players for taking the knee at their match on 5 December
Colchester's League Two game with Grimsby on the same day was preceded by fans booing the players' anti-racism gesture before kick-off.
The Essex club's owner Robbie Cowling said afterwards that fans who want to boo players taking the knee should not attend matches.
He said: "Those taking the knee, and supporting the taking of the knee, not only show their willingness to support the drive to eradicate racial oppression but force it to be a talking point even when it's uncomfortable.
Boos as players take a knee
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"Maybe those that booed on Saturday might now understand what this gesture means to our club and will at the very least remain silent during future games whilst the players continue to take the knee before each kick-off.
"Alternatively, they should just stay away from our club because anyone that still wants to boo now that I have explained the purpose and importance of the taking of the knee is not welcome at our club.
"I will be happy to refund anyone for the remaining value of their season permit if that is the reason they feel they can no longer attend our games."